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screening kit  (TaKaRa)


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    Structured Review

    TaKaRa screening kit
    Screening Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 583 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/differential+screening+kit/Clontech+PCR-Select+Differential+Screening+Kit/pm40248859-91-15-17
    Average 95 stars, based on 583 article reviews
    screening kit - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Identification of up-regulated transcripts during Pleurotus ostreatus primordium stage and characterization of PoALDH1.
    Article Snippet: Funding information Special Funds for the National Modern Agricultural Industry Technology System, Grant number: CARS-24; Science and Technology Project of Henan Province, Grant number: 162102110091 In order to isolate the differentially expressed genes in the primordium stage of Pleurotus ostreatus, the SSH cDNA library was constructed using the cDNA from dikaryotic mycelium stage as a driver and the cDNA from primordium stage as a tester.. Therewere 423 significantly differently expressed clones among 2055 positive clones after three times of reverse Northern blot differential screening.. After the repeated sequences being removed, 46 genes were identified which were putatively involved in cell rescue and defense, energy metabolism, transcription and protein regulation, membrane proteins, and signal transduction; 18 genes encoding hypothetical proteins with unknown function; 5 genes without any homology.

    Article Title: Molecular characterization of eliminated chromosomes in Hessian fly ( Mayetiola destructor (Say))
    Article Snippet: .. Suppressive subtractive hybridization (SSH) libraries were generated and screened using the PCR-Select cDNA subtraction kit and PCR-Select differential screening kit (Catalog #63791 and #637403, Clontech, Palo Alto, CA), modified upon the advice of the Clontech support staff by using genomic DNA as the starting material. ..

    Article Title: Molecular characterization of eliminated chromosomes in Hessian fly (Mayetiola destructor (Say)).
    Article Snippet: .. Suppressive subtractive hybridization (SSH) DNA libraries Suppressive subtractive hybridization (SSH) libraries were generated and screened using the PCR-Select cDNA subtraction kit and PCR-Select differential screening kit (Catalog #63791 and #637403, Clontech, Palo Alto, CA), modified upon the advice of the Clontech support staff by using genomic DNA as the starting material. ..

    Article Title: Radmis, a Novel Mitotic Spindle Protein that Functions in Cell Division of Neural Progenitors
    Article Snippet: .. SSH libraries were constructed using the PCR-SelectTM cDNA Subtraction Kit and PCR-SelectTM Differential Screening Kit (Clontech Takara Bio, Shiga, Japan), according to the manufacturer’s suggested conditions [ ]. .. Poly (A) + RNA (2.0 μg) from the stem cell culture and differentiated sister culture were purified using oligotex-dT30 Super (Takara Bio, Shiga, Japan), and used for synthesis of tester (St) and driver (Dif) cDNA, respectively.

    Article Title: Preeclampsia is Associated with Sex-Specific Transcriptional and Proteomic Changes in Fetal Erythroid Cells
    Article Snippet: Poly A + RNA purification was performed using Oligotex Direct mRNA mini kit (Qiagen) following the manufacturer’s instructions. .. Subtractive hybridization was carried out using the Clontech ® PCR-SelectTM Differential Screening kit (Takara Bio USA, Inc., Mountain View, CA 94043, USA) according to the manufacturer’s protocol. ..

    Article Title: GmFT2a , a Soybean Homolog of FLOWERING LOCUS T , Is Involved in Flowering Transition and Maintenance
    Article Snippet: .. An expressed sequence tag (EST), homologous to FT , was identified using the PCR-SelectTM Differential Screening Kit (Clontech). .. The full-length GmFT2a was cloned into the pGEM-T easy vector (Promega) using the FirstChoiceTM RLM-RACE Kit (Ambion).

    Article Title: Effects of salinity on the transcriptome of growing maize leaf cells point at cell-age specificity in the involvement of the antioxidative response in cell growth restriction
    Article Snippet: .. Dot-blot analysis was performed with a PCR-select differential screening kit (Clontech Laboratories Inc., Mountain View, USA). ..

    Isolation:

    Article Title: A Group D MAPK Protects Plants from Autotoxicity by Suppressing Herbivore-Induced Defense Signaling
    Article Snippet: The BPH ( Nilaparvata lugens ) colony was originally obtained from a rice field in Hangzhou, China, and maintained on TN1 rice seedlings (a rice variety that is susceptible to BPH) in a climate chamber at 26 ± 2°C, with a 12-h light phase (Philips, fluorescent tubes) and 65% relative humidity. .. Isolation of OsMAPK20-5 cDNA A subtractive library was constructed using a cDNA subtraction kit (TaKaRa, Cat.# 637401) and clones were identified using a differential screening kit (TaKaRa, Cat.# 637403) following the manufacturer’s instructions. ..

    Construct:

    Article Title: A Group D MAPK Protects Plants from Autotoxicity by Suppressing Herbivore-Induced Defense Signaling
    Article Snippet: The BPH ( Nilaparvata lugens ) colony was originally obtained from a rice field in Hangzhou, China, and maintained on TN1 rice seedlings (a rice variety that is susceptible to BPH) in a climate chamber at 26 ± 2°C, with a 12-h light phase (Philips, fluorescent tubes) and 65% relative humidity. .. Isolation of OsMAPK20-5 cDNA A subtractive library was constructed using a cDNA subtraction kit (TaKaRa, Cat.# 637401) and clones were identified using a differential screening kit (TaKaRa, Cat.# 637403) following the manufacturer’s instructions. ..

    Article Title: Radmis, a Novel Mitotic Spindle Protein that Functions in Cell Division of Neural Progenitors
    Article Snippet: .. SSH libraries were constructed using the PCR-SelectTM cDNA Subtraction Kit and PCR-SelectTM Differential Screening Kit (Clontech Takara Bio, Shiga, Japan), according to the manufacturer’s suggested conditions [ ]. .. Poly (A) + RNA (2.0 μg) from the stem cell culture and differentiated sister culture were purified using oligotex-dT30 Super (Takara Bio, Shiga, Japan), and used for synthesis of tester (St) and driver (Dif) cDNA, respectively.

    Clone Assay:

    Article Title: A Group D MAPK Protects Plants from Autotoxicity by Suppressing Herbivore-Induced Defense Signaling
    Article Snippet: The BPH ( Nilaparvata lugens ) colony was originally obtained from a rice field in Hangzhou, China, and maintained on TN1 rice seedlings (a rice variety that is susceptible to BPH) in a climate chamber at 26 ± 2°C, with a 12-h light phase (Philips, fluorescent tubes) and 65% relative humidity. .. Isolation of OsMAPK20-5 cDNA A subtractive library was constructed using a cDNA subtraction kit (TaKaRa, Cat.# 637401) and clones were identified using a differential screening kit (TaKaRa, Cat.# 637403) following the manufacturer’s instructions. ..

    Hybridization:

    Article Title: Molecular characterization of eliminated chromosomes in Hessian fly ( Mayetiola destructor (Say))
    Article Snippet: .. Suppressive subtractive hybridization (SSH) libraries were generated and screened using the PCR-Select cDNA subtraction kit and PCR-Select differential screening kit (Catalog #63791 and #637403, Clontech, Palo Alto, CA), modified upon the advice of the Clontech support staff by using genomic DNA as the starting material. ..

    Article Title: Molecular characterization of eliminated chromosomes in Hessian fly (Mayetiola destructor (Say)).
    Article Snippet: .. Suppressive subtractive hybridization (SSH) DNA libraries Suppressive subtractive hybridization (SSH) libraries were generated and screened using the PCR-Select cDNA subtraction kit and PCR-Select differential screening kit (Catalog #63791 and #637403, Clontech, Palo Alto, CA), modified upon the advice of the Clontech support staff by using genomic DNA as the starting material. ..

    Article Title: Preeclampsia is Associated with Sex-Specific Transcriptional and Proteomic Changes in Fetal Erythroid Cells
    Article Snippet: Poly A + RNA purification was performed using Oligotex Direct mRNA mini kit (Qiagen) following the manufacturer’s instructions. .. Subtractive hybridization was carried out using the Clontech ® PCR-SelectTM Differential Screening kit (Takara Bio USA, Inc., Mountain View, CA 94043, USA) according to the manufacturer’s protocol. ..

    Generated:

    Article Title: Molecular characterization of eliminated chromosomes in Hessian fly ( Mayetiola destructor (Say))
    Article Snippet: .. Suppressive subtractive hybridization (SSH) libraries were generated and screened using the PCR-Select cDNA subtraction kit and PCR-Select differential screening kit (Catalog #63791 and #637403, Clontech, Palo Alto, CA), modified upon the advice of the Clontech support staff by using genomic DNA as the starting material. ..

    Article Title: Molecular characterization of eliminated chromosomes in Hessian fly (Mayetiola destructor (Say)).
    Article Snippet: .. Suppressive subtractive hybridization (SSH) DNA libraries Suppressive subtractive hybridization (SSH) libraries were generated and screened using the PCR-Select cDNA subtraction kit and PCR-Select differential screening kit (Catalog #63791 and #637403, Clontech, Palo Alto, CA), modified upon the advice of the Clontech support staff by using genomic DNA as the starting material. ..

    Modification:

    Article Title: Molecular characterization of eliminated chromosomes in Hessian fly ( Mayetiola destructor (Say))
    Article Snippet: .. Suppressive subtractive hybridization (SSH) libraries were generated and screened using the PCR-Select cDNA subtraction kit and PCR-Select differential screening kit (Catalog #63791 and #637403, Clontech, Palo Alto, CA), modified upon the advice of the Clontech support staff by using genomic DNA as the starting material. ..

    Article Title: Molecular characterization of eliminated chromosomes in Hessian fly (Mayetiola destructor (Say)).
    Article Snippet: .. Suppressive subtractive hybridization (SSH) DNA libraries Suppressive subtractive hybridization (SSH) libraries were generated and screened using the PCR-Select cDNA subtraction kit and PCR-Select differential screening kit (Catalog #63791 and #637403, Clontech, Palo Alto, CA), modified upon the advice of the Clontech support staff by using genomic DNA as the starting material. ..

    Sequencing:

    Article Title: GmFT2a , a Soybean Homolog of FLOWERING LOCUS T , Is Involved in Flowering Transition and Maintenance
    Article Snippet: .. An expressed sequence tag (EST), homologous to FT , was identified using the PCR-SelectTM Differential Screening Kit (Clontech). .. The full-length GmFT2a was cloned into the pGEM-T easy vector (Promega) using the FirstChoiceTM RLM-RACE Kit (Ambion).



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